| Name | Position | Debut Team | Debut Date | CPL Team | | Michael Crudale | RHP | St. Louis | Apr 10, 2002 | Florence '98 |
| Ty Wigginton | 3B | New York (NL) | May 16, 2002 | Rocky Mount '97, Florence '98 |
| Kyle Snyder | RHP | Kansas City | May 1, 2003 | Raleigh '97 |
| Todd Wellemeyer | RHP | Chicago (NL) | May 15, 2003 | Thomasville '99 |
| David Aardsma | RHP | San Francisco | Apr 6, 2004 | Fayetteville '01 |
| Kevin Youkilis | 1B | Boston | May 15, 2004 | Florence '99 |
| Jason Dubois | OF | Chicago (NL) | May 19, 2004 | Rocky Mount '98 |
| Sam Narron | LHP | Texas | Jul 30, 2004 | Outer Banks '00 |
| Russ Adams | IF | Toronto | Sep 3, 2004 | Durham '00 |
| Dallas McPherson | 3B | Los Angeles (AL) | Sep 10, 2004 | Asheboro '99 |
| Ryan Speier | RHP | Colorado | Apr 4, 2005 | Florence '00 |
| Justin Verlander | RHP | Detroit | Jul 4, 2005 | Wilson '02 |
| Adam Greenberg | OF | Chicago (NL) | Jul 9, 2005 | Wilson '00 |
| Brad Eldred | 1B | Pittsburgh | Jul 22, 2005 | Wilson '01 |
| Ryan Zimmerman | 3B | Washington | Sep 1, 2005 | Peninsula '03 |
| Josh Rupe | RHP | Texas | Sep 16, 2005 | Peninsula '01 |
| Jordan Tata | RHP | Detroit | Apr 6, 2006 | Durham '02 |
| Bill Bray | LHP | Washington | Jun 3, 2006 | Asheboro '02 |
| Tom Mastny | RHP | Cleveland | Jul 30, 2006 | Wilson '01, Wilmington '02 |
| Brett Campbell | RHP | Washington | Sep 7, 2006 | Petersburg '01 |
| Joe Smith | RHP | New York (NL) | Apr 1, 2007 | Edenton '05 |
| Don Kelly | SS | Detroit | Apr 2, 2007 | Petersburg '00 |
| Mark Reynolds | 3B | Arizona | May 16, 2007 | Fayetteville '03 |
| Mark McLemore | LHP | Houston | May 24, 2007 | Florence '02 |
| Brett Carroll | OF | Miami | Jun 17, 2007 | Florence '03 |
| Justin Maxwell | OF | Washington | Sep 5, 2007 | Outer Banks '02 |
| John Bowker | OF | San Francisco | Apr 12, 2008 | Peninsula '02 |
| Tug Hulett | 2B | Seattle | Jul 12, 2008 | Peninsula '02 |
| Bobby Parnell | RHP | New York (NL) | Sep 15, 2008 | Thomasville '03 |
| A.J. Ellis | C | Los Angeles (NL) | Sep 15, 2008 | Durham '01, '02 |
| Landon Powell | C | Oakland | Apr 11, 2009 | Wilmington '01 |
| Ryan Sadowski | RHP | San Francisco | Jun 28, 2009 | Edenton '02 |
| Tyler Colvin | OF | Chicago (NL) | Sep 21, 2009 | Florence '04 |
| John Raynor | OF | Pittsburgh | Apr 8, 2010 | Wilmington '05 |
| Steven Tolleson | SS | Oakland | Apr 28, 2010 | Spartanburg '03 |
| Michael Kohn | RHP | Los Angeles (AL) | Jul 26, 2010 | Florence '05, Columbia '06 |
| Greg Holland | RHP | Kansas City | Aug 8, 2010 | Edenton '06 |
| Michael McKenry | C | Colorado | Sep 8, 2010 | Fayetteville '05 |
| Chris Hatcher | RHP/C | Miami | Sep 19, 2010 | Wilson '04, Wilmington '05 |
| Jerry Sands | OF | Los Angeles (NL) | Apr 18, 2011 | Spartanburg '06, Wilson '07, '08 |
| Cody Eppley | RHP | Texas | Apr 23, 2011 | Wilmington '07 |
| Evan Scribner | RHP | San Diego | Apr 26, 2011 | Edenton '06 |
| Brandon Guyer | OF | Tampa Bay | May 6, 2011 | Wilmington '05 |
| Andy Dirks | OF | Detroit | May 16, 2011 | Fayetteville '05 |
| David Carpenter | RHP | Houston | Jun 30, 2011 | Peninsula '06 |
| Scott Diamond | LHP | Minnesota | Jul 18, 2011 | Martinsville '06, '07 |
| Dave Sappelt | OF | Cincinnati | Aug 7, 2011 | Florence '06 |
| Brad Brach | RHP | San Diego | Aug 31, 2011 | Petersburg '06 |
| J.J. Hoover | RHP | Cincinnati | Apr 25, 2012 | Wilson '07 |
| D.J. Mitchell | RHP | New York (AL) | May 1, 2012 | Thomasville '06 |
| Kevin Mattison | OF | Miami | May 12, 2012 | Wilson '05, Edenton '07 |
| Adam Warren | RHP | New York (AL) | Jun 29, 2012 | New Bern '06 |
| Josh Edgin | LHP | New York (NL) | Jul 13, 2012 | Forest City '08, '09 |
| Carter Capps | RHP | Seattle | Aug 3, 2012 | Martinsville '09, Fayetteville '10 |
| Tom Layne | LHP | San Diego | Aug 14, 2012 | Martinsville '06 |
| David Lough | OF | Kansas City | Sep 1, 2012 | Florence '07 |
| Tom Koehler | RHP | Miami | Sep 5, 2012 | New Bern '06, '07 |
| Andrew Taylor | LHP | Los Angeles (AL) | Sep 27, 2012 | Wilson '06 |
| Phil Irwin | RHP | Pittsburgh | Apr 14, 2013 | Thomasville '08 |
| Justin Freeman | RHP | Cincinnati | Apr 17, 2013 | Edenton '07 |
| Vidal Nuno | LHP | New York (AL) | Apr 29, 2013 | Edenton '08 |
| Chris McGuiness | 1B | Texas | Jun 7, 2013 | Thomasville '08, '09 |
| Rob Wooten | RHP | Milwaukee | Jul 26, 2013 | Wilson 04, Wilmington '06 |
| Heath Hembree | RHP | San Francisco | Sep 3, 2013 | Forest City '08, '09 |
| Aaron Barrett | RHP | Washington | Mar 31, 2014 | Thomasville '09 |
| Ian Thomas | LHP | Atlanta | Mar 31, 2014 | Peninsula '08 |
| Kevin Quackenbush | RHP | San Diego | Apr 25, 2014 | Fayetteville '08 |
| Eric Campbell | 1B | New York (NL) | May 10, 2014 | Martinsville '07 |
| Dallas Beeler | RHP | Chicago (AL) | Jun 28, 2014 | Florence '10 |
| Vince Belnome | 1B | Tampa Bay | Jul 3, 2014 | Forest City '09 |
| Chris Taylor | SS | Seattle | Jul 24, 2014 | Morehead City '10 |
| Billy Burns | CF | Oakland | Jul 28, 2014 | Edenton '10 |
| Buck Farmer | RHP | Detroit | Aug 13, 2014 | Peninsula '10 |
| Chris Bassitt | RHP | Chicago (AL) | Aug 30, 2014 | Edenton '10 |
| Spencer Patton | RHP | Texas | Sep 4, 2014 | Spartanburg '07, Forest City '08, '09 |
| Asher Wojciechowski | RHP | Houston | Apr 9, 2015 | Thomasville '09 |
| John Cornely | RHP | Atlanta | Apr 29, 2015 | Outer Banks '08, '09, '10 |
| Ken Roberts | LHP | Colorado | May 3, 2015 | Edenton '08 |
| Mike Wright | RHP | Baltimore | May 17, 2015 | Asheboro '09 |
| Shawn Armstrong | RHP | Cleveland | Aug 9, 2015 | Morehead City '10, '11 |
| Keith Hessler | LHP | Arizona | Aug 9, 2015 | Outer Banks '09 |
| Tyler White | 1B/DH | Houston | Apr 5, 2016 | Gastonia '11, '12 |
| Chad Girodo | LHP | Toronto | Apr 22, 2016 | Wilson '11 |
| Tim Adleman | RHP | Cincinnati | May 1, 2016 | Wilson '08, '09 |
| Pat Dean | LHP | Minnesota | May 11, 2016 | High Point-Thomasville '08, '09 |
| Layne Somsen | RHP | Cincinnati | May 14, 2016 | Fayetteville '12 |
| Ryan Garton | RHP | Tampa Bay | May 26, 2016 | Columbia '10 |
| Mike Mayers | RHP | St. Louis | Jul 24, 2016 | High Point-Thomasville '11 |
| Danny Barnes | RHP | Toronto | Aug 2, 2016 | Petersburg '08 |
| Chad Pinder | 2B | Oakland | Aug 20, 2016 | Peninsula '11 |
| Joey Wendle | 2B | Oakland | Aug 31, 2016 | Edenton '11 |
| Jake Smith | RHP | San Diego | Sep 7, 2016 | Florence '10 |
| Jharel Cotton | RHP | Oakland | Sep 7, 2016 | Peninsula '10, '12 |
| Kevin McCarthy | RHP | Kansas City | Sep 9, 2016 | Edenton '12 |
| Emilio Pagan | RHP | Seattle | May 3, 2017 | Wilmington '11, Forest City '12 |
| Jordan Jankowski | RHP | Houston | May 24, 2017 | High Point-Thomasville '09, '10, '11 |
| Adam Engel | OF | Chicago (AL) | May 27, 2017 | Florence '11 |
| Seth Frankoff | RHP | Chicago (NL) | Jun 9, 2017 | Wilmington '07 |
| Phil Maton | RHP | San Diego | Jun 11, 2017 | High Point-Thomasville '14 |
| Max Povse | RHP | Seattle | Jun 22, 2017 | Asheboro '12 |
| Tyler Webb | LHP | New York (AL) | Jun 24, 2017 | Columbia '10 |
| Mike Tauchman | OF | Colorado | Jun 27, 2017 | Fayetteville '12 |
| Kevin Shackelford | RHP | Cincinnati | Jun 29, 2017 | Petersburg '08 |
| Adam Kolarek | RHP | Tampa Bay | Jun 29, 2017 | Outer Banks '09 |
| Chris O'Grady | LHP | Miami | Jul 8, 2017 | Wilmington '11 |
| Caleb Smith | LHP | New York (AL) | Jul 17, 2017 | Morehead City '11 |
| Troy Scribner | RHP | Los Angeles (AL) | Jul 29, 2017 | Edenton '12 |
| Chris Rowley | RHP | Toronto | Aug 12, 2017 | Peninsula '12 |